Ultrasonic assisted extraction of chlorogenic acid from papaya

Abstract

The extraction reagent used in the common chlorogenic acid extraction methods can not effectively dissolve chlorogenic acid in papaya, resulting in low extraction rate and purity of chlorogenic acid. To solve the above problems, an ultrasonic assisted extraction method of chlorogenic acid from naked papaya was proposed. The medicinal, ornamental and edible values of papaya were analysed. Taking the six frequency slit ultrasonic extraction device as the core, the ultrasonic extraction device was designed. The ultrasonic assisted technology was introduced, and the crude chlorogenic acid was obtained by using the extraction reagent through the processes of dissolution, centrifugation, extraction and drying. The content of chlorogenic acid was determined by UV-Vis spectrophotometer and liquid chromatography. Crude chlorogenic acid was dissolved in ethanol and passed through a macroporous resin layer to obtain a saturated adsorption resin. The resin was placed in the desorption solution and allowed to stand to obtain a purified chlorogenic acid solution. The chlorogenic acid solute was rotated, evaporated and dried to complete the extraction of purified chlorogenic acid from papaya. The experimental results of the extraction method designed in this paper show that the extraction time is 20min, the extraction rate is 1.95%, the dosage of extractant is 50ml, and the extraction purity is 63.14%. It has practical value to ensure its efficient application in the research of food industry, pharmaceutical industry and daily cosmetics.